A minimized designer protein for facile biofabrication of ZnS:Mn immuno-quantum dots.
نویسندگان
چکیده
A minimized protein consisting of a linear ZnS-binding peptide fused to an antibody-binding domain supports the one-step aqueous synthesis of Mn-doped ZnS nanocrystals that exhibit smaller size, brighter fluorescence and improved antibody-binding relative to those made with the original designer protein.
منابع مشابه
Microbial Uptake, Toxicity, and Fate of Biofabricated ZnS:Mn Nanocrystals
Despite their importance in nano-environmental health and safety, interactions between engineered nanomaterials and microbial life remain poorly characterized. Here, we used the model organism E. coli to study the penetration requirements, subcellular localization, induction of stress responses, and long-term fate of luminescent Mn-doped ZnS nanocrystals fabricated under "green" processing cond...
متن کاملBiocompatible ZnS:Mn quantum dots for reactive oxygen generation and detection in aqueous media
ABSTRACT We report here the versatility of Mn-doped ZnS quantum dots (ZnS:Mn QDs) synthesized in aqueous medium for generating reactive oxygen species and for detecting cells. Our experiments provide evidence leading to the elimination of Cd-based cores in CdSe/ZnS systems by substitution of Mn-doped ZnS. Advanced electron microscopy, X-ray diffraction, and optical spectroscopy were applied to ...
متن کاملNS-ZnS:Mn, ZnS:Mn/Si3N4 multilayers with thicknesses of 1.9– 3.5 nm for ZnS were prepared by a rf-magnetron sputtering method. From the results of grazing incidence X-ray reflectometry and X-ray diffractmetry, formation of ZnS:Mn nanocrystals in the ZnS
We have developed a new type of a thin-film electroluminescence (TFEL) device with nanostructured (NS)-ZnS:Mn utilizing its enhanced luminescent efficiency due to the quantum confinement (QC) effects. As NS-ZnS:Mn, ZnS:Mn/Si3N4 multilayers with thicknesses of 1.9– 3.5 nm for ZnS were prepared by a rf-magnetron sputtering method. From the results of grazing incidence X-ray reflectometry and X-ra...
متن کاملTuning the Luminescence of CdS Quantum Dots by a Simple Method
In this report, we present a facile approach for the synthesis of luminescent CdS and CdS:Mn+2 nanocrystals by reaction of CdSO4 and Na2S2O3 in the presence of thioglycerol (C3H8O2S) as capping agent. The influence of various experimental variables including, pH values and percentage of dopant, on the growth...
متن کاملA comparative study about toxicity of CdSe quantum dots on reproductive system development of mice and controlling this toxicity by ZnS coverage
Objective(s): Medicinal benefits of quantum dots have been proved in recent years but there is little known about their toxicity especially in vivo toxicity. In order to use quantum dots in medical applications, studies ontheir in vivo toxicity is important. Materials and Methods:CdSe:ZnS quantum dots were injected in 10, 20, and 40 mg/kg doses to male mice10 days later, mice were sacrificed ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemical communications
دوره 51 17 شماره
صفحات -
تاریخ انتشار 2015